Rapid laboratory test for Haemonchus in worm egg counts

The lectin binding assay is a laboratory test that rapidly indicates the proportion of Haemonchus contortus (Barbers Pole worm) eggs in faecal samples submitted for worm egg counts. Eggs are isolated from the sample through a series of steps (including centrifuging) and then stained with a dye that binds specifically to Haemonchus eggs. Under a microscope capable of detecting fluorescent samples, the labelled eggs stand out compared to eggs from other worm species, and can be rapidly counted to indicate the percentage of Haemonchus.

The ability to indicate the level of Haemonchus on the same day that an egg count is conducted allows immediate treatment if necessary, and avoids the week-long delay necessary with the existing larval culture method. Better treatment decisions can be made with immediate and objective information on the Haemonchus count, increasing the benefits and likely uptake of worm egg counting. However, this procedure requires specific laboratory equipment and is most appropriate where Barbers Pole worm is a major risk.

Benefits:

• Same-day indication of the percentage of Haemonchus contortus in a worm egg count sample;
• More objective information for drenching decisions compared to worm egg counts alone.

Recommended users: Worm egg counting laboratories, advisors & consultants, teachers & educators

Laboratories interested in using this test contact Dr Brown Besier via email: brown.besier@agric.wa.gov.au

Choose from the tabs below to find more detailed information.